Journal: Clinical chemistry
Article Title: Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR.
doi: 10.1373/clinchem.2015.239137
Figure Lengend Snippet: Fig. 3. Fetal RHD genotyping results from maternal plasma samples. The concentration (copies per microliter) (plus SD) was identified for both target regions (RHD5 and RHD7) and the reference region (AGO1). The presence or absence of the target regions were used to determine fetal status (RHD+ or RHD−, respectively). (A), Maternal samples collected in EDTA tubes (n = 22). (B), Maternal samples collected in Streck BCTs (n = 24). The same controls are represented in both graphs. The control nonmaternal cfDNA RHD-positive sample (399X) exhibited ratios of 0.51 and 0.47 for RHD5/AGO1 and RHD7/AGO1, respectively.
Article Snippet: The use of Streck® Cell-Free DNATM blood collection tubes (BCTs) instead of conventional EDTA tubes has been shown to increase the proportion of cffDNA (21 ).
Techniques: Clinical Proteomics, Concentration Assay, Control